论文部分内容阅读
目的检测喉癌组织中Dickkopf-1的表达,分析其表达与喉癌临床病理因素之间的关系及探讨与喉癌发生、发展的关系及临床的潜在价值。方法采用免疫组化SP法检测56例喉癌组织和20例喉部正常黏膜组织中的Dickkopf-1的表达情况。结果 Dickkopf-1蛋白在喉癌组织中的阳性表达率为44.64%,明显低于喉部正常黏膜组织的90.00%。Dickkopf-1表达在有淋巴结转移组为15.79%,明显低于无淋巴结转移组的64.86%。在临床Ⅲ~Ⅳ期组阳性表达率为27.03%,明显低于临床Ⅰ~Ⅱ期组的84.21%,低分化组为15.38%低于高分化的63.16%及中分化组的58.33%,差异均有统计学意义(P均<0.05)。Dickkopf-1在喉癌组织中的表达与临床分型、肿瘤大小、吸烟量、年龄和性别无关(P>0.05)。结论 Dickkopf-1在喉癌组织中的阳性表达率明显低于喉部正常黏膜组织,提示Dickkopf-1表达可能与淋巴结转移、临床分期和病理分级有关。
Objective To detect the expression of Dickkopf-1 in laryngeal carcinoma and to analyze its relationship with the clinicopathological factors of laryngeal carcinoma and its relationship with the occurrence and development of laryngeal carcinoma and its clinical value. Methods Immunohistochemical SP method was used to detect the expression of Dickkopf-1 in 56 cases of laryngeal carcinoma and 20 cases of normal laryngeal mucosa. Results The positive rate of Dickkopf-1 protein in laryngeal carcinoma was 44.64%, which was significantly lower than that of normal laryngeal mucosa (90.00%). Dickkopf-1 expression in lymph node metastasis group was 15.79%, significantly lower than 64.86% without lymph node metastasis group. The positive expression rate in clinical stage Ⅲ ~ Ⅳ group was 27.03%, which was significantly lower than 84.21% in clinical stage Ⅰ ~ Ⅱ group, 15.38% in poorly differentiated group, 63.16% in well differentiated group and 58.33% in moderately differentiated group There was statistical significance (all P <0.05). The expression of Dickkopf-1 in laryngeal carcinoma was not related to clinical classification, tumor size, smoking amount, age and gender (P> 0.05). Conclusions The positive expression rate of Dickkopf-1 in laryngeal carcinoma was significantly lower than that in normal laryngeal mucosa, suggesting that Dickkopf-1 expression may be related to lymph node metastasis, clinical stage and pathological grade.