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目的:对传统中药蛇床子基原植物及其近缘物种的ITS2条形码序列进行分析,探讨药用植物蛇床子的分子鉴定方法。方法:采用PCR技术扩增蛇床ITS2基因片段,进行双向测序,运用CodonCodeAligner拼接后,用MEGA5.0软件进行相关数据分析,构建NJ树。应用Schultz等建立的ITS2数据库及其网站预测ITS2二级结构。结果:蛇床与其近缘物种ITS2序列间存在明显差异,基于ITS2序列的NJ树及其ITS2二级结构均可以将蛇床及其近缘物种区分开。结论:ITS2可有效地鉴别蛇床及其近缘物种。
OBJECTIVE: To analyze the ITS2 barcode sequence of the traditional Chinese medicine Cnidium and its related species, and to explore the molecular identification method of the medicinal plant Cnidium. Methods: ITS2 gene fragment was amplified by PCR and sequenced by two-way sequencing. After splicing with CodonCodeAligner, MEGA5.0 software was used to analyze the related data to construct NJ tree. The ITS2 database established by Schultz et al. And its website are used to predict ITS2 secondary structure. Results: There were significant differences in the ITS2 sequences between the snake bed and its related species. The NJ tree and its ITS2 secondary structure based on ITS2 sequences could distinguish the snake bed and its related species. Conclusion: ITS2 can effectively identify the snake bed and its related species.