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为了检测转DREB(干旱应答元件结合蛋白)基因香花槐(Robinia pseudoacacia cv idaho)枯落物对土壤生态的影响,模拟林木落叶与土壤互作的自然过程,以转DREB基因及非转基因香花槐枝叶为材料,将枝叶与土壤以一定比例混匀,统计转基因香花槐和非转基因香花槐枝叶降解过程中土壤主要微生物群落数量的变化动态,通过探讨土壤中卡那抗性细菌的菌落变化来了解转化载体上抗性标记基因nptII的漂移,从而了解外源基因环境释放的安全性。结果表明,在培养初期(3 d)和第54 d,转基因香花槐落叶细菌菌落数小于非转基因香花槐落叶的细菌菌落数,但无显著差异(p>0.05)。枝叶土壤中放线菌菌落数量在前21 d高低波动,但总体无显著差异(p>0.05)。在处理第3 d,含转基因香花槐枝叶的土壤霉菌菌落数量高于非转基因香花槐枝叶土壤;在第17 d和第36 d含非转基因香花槐枯落物土样霉菌菌落数量高于转基因土壤,且第17 d差异显著(p<0.05)。转基因植株和非转基因植株对土壤中3种微生物种群均没有显著影响。此外,转基因枝叶处理土壤的卡那抗性细菌菌落数量整体低于其非转基因枝叶处理的土样,但差异不显著(p>0.05)。研究表明,在实验室培养条件下,转基因香花槐枯落物未对土壤主要微生物产生显著影响。
In order to detect the influence of DREB (Roles of Drought-Responsive Element Binding Protein) gene on soil ecology and simulate the natural process of tree deciduous leaf-soil interaction with DREB gene and non-genetically engineered Fraxinus mandshurica As the material, the foliage and the soil were mixed in a certain proportion, and the changes of the quantity of the main microbial communities in the soil during the degradation of the branches and the leaves of the transgenic A. pseudoacacia and the non-transgenic A. fragrans were counted. The transformation was analyzed by examining the colony changes of the karna- Drift of the marker gene nptII on the vector to understand the safety of the exogenous gene release. The results showed that the number of bacterial colonies in the locusts was less than that in the non-locust cultivars (p> 0.05) at the initial (3 d) and day 54 d of cultivation. The number of actinomycete colonies in the leaves and leaves fluctuated in the first 21 days, but there was no significant difference (p> 0.05). On the third day, the number of soil mold colonies with transgenic A. fragrans branches was higher than that of the non-transgenic A. citriana leaves. The number of mold-like colonies with non-transgenic A. sinensis litter on the 17th and 36th days was higher than that of the transgenic soil , And the difference was significant on the 17th day (p <0.05). Both transgenic and non-transgenic plants had no significant effect on the three microbial populations in soil. In addition, the total number of kanamycin-resistant bacterial colonies treated with transgenic shoots was lower than that of non-transgenic shoots, but the difference was not significant (p> 0.05). Studies have shown that under laboratory culture conditions, the R. solanacearum litter does not significantly affect the main soil microorganisms.