作者采用经γ射线辐射的墨西哥利什曼原虫,体外感染巨噬细胞,选择出15,000rad剂量辐射虫体免疫动物,获得较好效果。用BALB/c鼠48只进行免疫实验,同种原虫攻击。按攻击部位损伤大小、病变结节重量及其内含原虫数等三项指标衡量免疫效果。免疫组与对照组自攻击感染6周后,三项指标均出现显著差异,说明免疫鼠具有明显的保护力,抗体滴度与保护力之间未出现吻合关系。 The authors used Leishmania mexicana irradiated by γ-rays to infect macrophages in vitro, and chose 15,000rad doses of radiation to immunize animals to get better results. 48 BALB / c mice were immunized, allogeneic protozoal attack. According to the size of the lesion at the site of attack, the weight of diseased nodules and the number of protozoa contained therein, the immunological effects were measured. Immunization group and control group self-attack infection after 6 weeks, the three indicators were significantly different, indicating that immunized mice with significant protection, antibody titers and protection did not occur between the anastomosis.