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目的 探讨原发性肺部恶性肿瘤与慢性阻塞性肺部疾病 (COPD)患者外周血淋巴细胞亚群及NK细胞水平测定的临床价值。方法 应用单克隆抗体 -碱性磷酸酶抗碱性磷酸酶法 (APAAP法 )测定 60例COPD病人 ,5 0例原发性肺部恶性肿瘤病人和 40例正常对照组CD3 + ,CD4+ ,CD8+ ,CD19+ 及NK细胞的百分数 ,计算CD4+ /CD8+ 比值。结果 原发性肺部恶性肿瘤组、COPD组的CD3 + ,CD4+ ,CD4+ /CD8+ ,CD19+ 明显降低 ,而CD8明显增高 ,与对照组比较均有显著差异 (P <0 0 1) ;原发性肺部恶性肿瘤组的NK细胞的百分数降低 ,与对照组比较有显著差异 (P <0 0 1) ,COPD组NK表达与对照组比较无显著差异 (P >0 0 5 )。原发性肺部恶性肿瘤组与COPD组的CD3 + ,CD4+ ,CD8+ 均无明显差异 (P >0 0 5 ) ,而NK ,CD19+ 有差异 (P <0 0 1)。结论 原发性肺部恶性肿瘤组和COPD组存在细胞免疫功能紊乱和失调 ,临床上应不失时机的应用免疫调节剂
Objective To investigate the clinical value of detecting peripheral blood lymphocyte subsets and NK cells in patients with primary pulmonary malignancies and chronic obstructive pulmonary disease (COPD). Methods 60 cases of COPD, 50 cases of primary lung cancer and 40 cases of normal control were measured by APAAP method. The levels of CD3 +, CD4 +, CD8 + CD19 + and NK cell percentage, calculated CD4 / CD8 ratio. Results In primary lung cancer group, the levels of CD3 +, CD4 +, CD4 + / CD8 + and CD19 + in COPD group were significantly lower than those in control group (P <0.01) The percentage of NK cells in the lung cancer group was significantly lower than that in the control group (P <0.01). There was no significant difference in the NK expression between the COPD group and the control group (P> 0.05). There was no significant difference in CD3 +, CD4 +, CD8 + between primary lung cancer group and COPD group (P> 0.05), but there was difference between NK and CD19 + group (P <0.01). Conclusions There are cellular immune dysfunction and disorder in the primary lung cancer group and COPD group, and the immunomodulatory agent