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目的研究肺癌肿瘤抑制因子-1(TSLC1)在喉鳞癌组织和癌旁正常组织的表达,探讨TSLC1与喉鳞癌的相关性,以期为喉癌的病因、诊断及治疗提供理论依据。方法取喉鳞状细胞癌组织标本蜡块45例,其中8例有淋巴结转移。取20例癌旁正常组织标本蜡块作为对照。应用免疫组化PV-6000二步法测定二者中TSLC1的表达,分析TSLC1的表达水平与各临床病理参数的关系。结果用SPSS 10.0统计软件进行统计学分析。结果 TSLC1在喉鳞癌标本中的阳性表达率为17/45(37.8%),明显低于癌旁正常组织中TSLC1的阳性表达率15/20(75.0%),二者比较差异有统计学意义(χ~2=7.68,P<0.01)。TSLC1的表达下降与喉鳞癌的临床分期、分化程度、淋巴结转移情况差异有统计学意义(χ~2=4.15、6.11、4.11,P<0.05),而与年龄、发病部位及性别差异无统计学意义(χ~2=0.25、0.09、1.0,P>0.05)。结论 TSLC1在喉鳞癌中表达下降,且与喉鳞癌的临床分期、分化程度、淋巴结转移情况有相关性。TSLC1在喉鳞癌中的表达下降可能与喉鳞癌的发生和侵袭有关,但需扩大样本进一步研究。
Objective To study the expression of TSLC1 in laryngeal squamous cell carcinoma and adjacent normal tissues and to explore the correlation between TSLC1 and laryngeal squamous cell carcinoma so as to provide a theoretical basis for the etiology, diagnosis and treatment of laryngeal cancer. Methods Forty-five paraffin-embedded specimens of laryngeal squamous cell carcinoma were obtained, of which 8 had lymph node metastasis. Take 20 cases of paraffin adjacent normal tissue specimens as a control. Immunohistochemistry PV-6000 two-step method to determine the expression of TSLC1, TSLC1 expression levels and the relationship between the various clinical and pathological parameters. Results SPSS 10.0 statistical software for statistical analysis. Results The positive rate of TSLC1 in laryngeal squamous cell carcinoma was 17/45 (37.8%), which was significantly lower than that of TSLC1 in normal tissues (15/20, 75.0%), the difference was statistically significant (χ ~ 2 = 7.68, P <0.01). There was a significant difference between the decreased expression of TSLC1 and the clinical stages, differentiation and lymph node metastasis of laryngeal squamous cell carcinoma (χ ~ 2 = 4.15,6.11,4.11, P <0.05), but no difference with age, incidence of disease and gender Significance (χ ~ 2 = 0.25,0.09,1.0, P> 0.05). Conclusion The expression of TSLC1 is down-regulated in laryngeal squamous cell carcinoma and is correlated with the clinical stage, differentiation and lymph node metastasis of laryngeal squamous cell carcinoma. The decreased expression of TSLC1 in laryngeal squamous cell carcinoma may be related to the occurrence and invasion of laryngeal squamous cell carcinoma, but the samples need to be expanded for further study.