目的　比较四氯化碳 (CCl4)法和免疫血清 (PS)法两种肝纤维化制模方法在肝纤维化形成进程中时效关系和病理学特征。方法　采用CCl4和PS 10周法制作SD大鼠肝纤维化模型 ,分别观察在造模第 6、10、14、2 0周时肝脏组病学特征 ,Masson三色染色和计算机图像分析系统进行分析。结果　CCl4法于制模第 6周即可见肝脏假小叶形成 ,于第 10周始最为典型 ;随着假小叶的形成和造模方法停止 ,肝脏病理结构变化趋于稳定 ;肝细胞脂肪变性尤为突出 ,与模型制作进程相平行。PS法于造模第 10周即造模结束时方见纤维间隔形成 ,造模虽已停止但肝纤维化进程加速完成 ,肝组织分隔严重 ,假小叶形成较多并进展到第 2 0周 ;制模自始至终 ,肝细胞未见明显脂肪变性。结论　CCl4和PS两种制模方法在肝纤维化形成进程中所表现出的时效关系和病理特征有所不同 ,结合使用两种方法研究肝纤维化更为合理 ;以抗肝纤维化干预因子进行干预研究时 ,干预因子的持续时间宜延长至肝纤维化形成之高峰期或之后 ,持续时间至少 14周 Objective To compare the time-effect relationship and pathological characteristics of two methods of liver fibrosis, including CCl4 and immune sera (PS), in the process of hepatic fibrosis. Methods The hepatic fibrosis model of SD rats was made by CCl4 and PS for 10 weeks. The liver histological features, Masson trichrome staining and computerized image analysis system were observed at the 6th, 10th, . Results The CCl4 method was used to detect the formation of hepatic pseudolobules at the 6th week of modeling. At the beginning of 10th week, the CCl4 method was most typical. With the formation of pseudolobules and the stopping of modeling methods, the pathological changes of hepatic tissues tended to be stable. , Parallel to the model making process. PS method in the first 10 weeks of modeling that is the end of the model to see the fiber interval formed, although the modeling has stopped but the accelerated progress of liver fibrosis, severe liver segregation, pseudolobule formation and progress to the 20th week; Throughout the model, liver cells showed no significant fatty degeneration. Conclusions Both CCl4 and PS are different in age-related and pathological characteristics in the process of hepatic fibrosis. It is more reasonable to combine two methods to study hepatic fibrosis. Anti-hepatic fibrosis intervention factors Intervention study, the duration of the intervention factor should be extended to the peak or after the formation of liver fibrosis, the duration of at least 14 weeks