本研究用玉米小斑菌(Helminthosporium maydis)为试材系统地研究了影响离体植物根冠细胞测定法的诸因素,对Hawes等(1982)的根冠细胞法进行了全面改进,提出了改良的根冠细胞测定法及其关键技术。这种方法简便,快速、精确,在作物品种抗性测定,生理小种,毒素及其它应用等方面具有广阔的应用前景。 应用抗病品种是防治植物病害最经济有效的措施,在培育抗病品种及测定大量品种的抗病性中,如何进一步提高测定的精确度、 In this study, we used Helminthosporium maydis as a test material to systematically study the factors influencing the measurement of root-and-canocytes in vitro. The method of root-crown cells was improved by Hawes et al. (1982) Root Crown Cell Assay and Its Key Techniques. The method is simple, rapid and accurate, and has broad application prospects in crop variety resistance testing, physiological races, toxins and other applications. The application of disease-resistant varieties is the most economical and effective measure to control plant diseases. In cultivating resistant varieties and measuring the disease resistance of a large number of varieties, how to further improve the accuracy of the determination,