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1目的 了解山东省 1型人类免疫缺陷病毒 (HIV- 1)的分子流行病学特征。 2方法 应用套式聚合酶链反应扩增 8例 HIV- 1前病毒基因组的 env基因 C2 - V 3区 ,扩增产物纯化后与 p GEM- T载体连接 ,克隆于大肠杆菌中 ,提取阳性克隆的重组质粒进行测序 ,用 PHYL IP软件分析核苷酸及氨基酸序列。 3结果 8株 HIV - 1毒株V3环顶端的四肽基序均为 GPGR,氨基酸变异不显著 ,各株间基因离散率为 1.0 %～ 5 .2 % ,侧翼区碱基的变异频率明显高于 V3区 ,并发现 1例毒株出现双 V 3环现象。 4结论 山东省的 HIV - 1分离株以 B亚型 HIV- 1毒株为主 ,同时发现的双 V3环变异现象 ,可能是 HIV - 1毒株逃避宿主免疫系统攻击的一种新模式。
1 Objective To understand the molecular epidemiological characteristics of human immunodeficiency virus type 1 (HIV-1) in Shandong Province. 2 Methods The env gene C2 - V 3 region of 8 HIV - 1 provirus genomes was amplified by nested polymerase chain reaction. The amplified product was purified and ligated into p GEM - T vector. The amplified product was cloned into E. coli and the positive clones were extracted The recombinant plasmids were sequenced and the nucleotide and amino acid sequences were analyzed using PHYL IP software. 3 Results The tetrapeptide motifs of the top of V3 loop of the 8 HIV - 1 strains were all GPGR with no obvious amino acid variation. The genetic divergence rate was 1.0% ~ 5.2% in each strain, and the mutation frequency of flanking region was significantly higher In the V3 region, 1 strain was found to have double V3 ring phenomenon. CONCLUSION: The HIV - 1 isolates in Shandong Province are mainly B - type HIV - 1 strains. The simultaneous discovery of the double V3 loop variation may be a new model for HIV - 1 strains to evade host immune system attack.