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目的探讨RUNX3基因及蛋白表达在锯齿状病变发生与癌变途径中的作用。方法应用序列特异引物-聚合酶链式反应(PCR-SSP)法研究50例锯齿状腺瘤(SA)中RUNX3(rs2236851,C/T)的多态性,其中包括传统锯齿状腺瘤(TSA)40例,广基锯齿状腺瘤/息肉(SSA/P)10例以及20例正常大肠组织;并从SA中抽取30例进行免疫组化染色,用IPP(Image pro plus)图像分析系统进行免疫组化阳性表达的半定量分析,分析基因型与RUNX3表达之间的关系。结果 SA试验组T等位基因的频率(18.8%)明显高于正常对照组(5.0%),差异有统计学意义(P<0.05);TC基因型SA患者的RUNX3平均吸光度(0.27±0.16)较CC基因型患者的平均吸光度(0.28±0.18)明显降低(P<0.05)。结论 RUNX3基因(rs2236851)位点可能与SA密切相关。
Objective To investigate the role of RUNX3 gene and protein in the pathogenesis of serrated lesions. Methods The polymorphisms of RUNX3 (rs2236851, C / T) in 50 cases of serrated adenomas (SA) were studied by PCR-SSP method, including the traditional serrated adenomas (TSA ), 40 cases of broad-based serrated adenoma / polyps (SSA / P) in 10 cases and 20 cases of normal colorectal tissues. Thirty cases of SA were selected for immunohistochemical staining and image pro plus (IPP) image analysis system Semi-quantitative analysis of immunohistochemical positive expression, analysis of the relationship between genotypes and RUNX3 expression. Results The frequency of T allele in SA group (18.8%) was significantly higher than that in normal control group (5.0%) (P <0.05). The mean RUNX3 absorbance (0.27 ± 0.16) Compared with CC genotype, the average absorbance (0.28 ± 0.18) was significantly lower (P <0.05). Conclusion The RUNX3 gene (rs2236851) may be closely related to SA.