以常压层析系统纯化的碱性脂肪酶为出发蛋白 ,采用高效反相色谱对其进一步分离纯化 ,达到N末端氨基酸测序及肽谱分析所需的纯度 .用电喷雾质谱测得脂肪酶的相对分子质量为2 72 1 7± 1 .对该脂肪酶的胰蛋白酶水解条件及肽段的相对分子质量等进行了研究 . The crude lipase purified by atmospheric chromatography was taken as the starting protein and further purified by high performance reversed-phase chromatography to achieve the purity required for N-terminal amino acid sequencing and peptide mapping.Lipase was determined by electrospray ionization mass spectrometry The relative molecular mass was 2 72 1 7 ± 1. The trypsin hydrolysis conditions of the lipase and the relative molecular mass of peptides were studied.