论文部分内容阅读
用理化方法制得链球菌G蛋白(SPG)-辣根过氧化物酶及SPG-胶体金探针,并将其用于常规病理组织切片、电镜包埋后、包埋前及冰冻超薄切片的抗原定位。组织化学染色显示,SPG定位准确、标记密度高,背景清晰,显著优于经典的葡萄球菌A蛋白(SPA)。研究结果表明,SPG的一个最重要的优点就是在免疫组化染色条件下仍有着比SPA更稳定、更可靠、更强的IgG亲合力,尤其适用于单克隆抗体,可获得更为理想的标记结果,因而是一种能用于多种不同条件组织化学染色的新型、高效免疫学检测剂。
Streptococcus mutans G protein (SPG) - horseradish peroxidase and SPG-colloidal gold probe were prepared by physical and chemical methods, and used for routine histological sections, electron microscope embedding, before embedding and frozen ultrathin section Antigen localization. Histochemical staining showed that SPG possesses accurate localization, high labeling density and clear background, which is significantly better than that of classical staphylococcal protein A (SPA). The results show that one of the most important advantages of SPG is that it still has a more stable, more reliable and stronger IgG affinity than SPA under immunohistochemical staining conditions, and is especially suitable for monoclonal antibodies for a more desirable marker As a result, it is a novel, highly efficient immunological detector that can be used for histochemical staining in many different conditions.