【摘 要】
：
利用生物信息学手段,以期获得蚯蚓纤溶酶F-Ⅰ-0组分的基因.根据从粉正蚓(Lumbricus rubellus)中分离的F-Ⅰ-0组分的N端氨基酸序列VVGGSDTTIGQYPHQL,利用DNAMAN软件通过电子克
论文部分内容阅读
利用生物信息学手段,以期获得蚯蚓纤溶酶F-Ⅰ-0组分的基因.根据从粉正蚓(Lumbricus rubellus)中分离的F-Ⅰ-0组分的N端氨基酸序列VVGGSDTTIGQYPHQL,利用DNAMAN软件通过电子克隆方法,从Lumbricidae 的dbEST中获得该组分的核酸序列信息,设计特异引物, 经过RT-PCR,成功地从赤子爱胜蚓(Eisenia foetida)中克隆到一条蚯蚓纤溶酶新基因,命名为EfP-0.EfP-0基因全长678bp, 编码225个氨基酸的成熟肽,属丝氨酸蛋白酶,胰蛋白酶家族,与F-Ⅰ-0组分的氨基酸组成非常接近.BLAST证明,EfP-0与已报道的蚯蚓纤溶酶基因之间的相似性均低于40%,因此为蚯蚓纤溶酶中的一个新基因,GenBank 登录号为DQ836917.构建的pMAL-c2x-EfP-0重组质粒,在大肠杆菌TB1中获得融合蛋白MBP- EfP-0的可溶性表达,表达产物有酪蛋白平板溶解活性.
其他文献
Background An important aspect of Alzheimers disease (AD) is loss or impairment of cholinergic neurons. It is controversial whether there is a similar cholinerg
Background Extracellular matrix (ECM) orchestrates cell behaviour including growth, death, apoptosis, adhesion, migration, and invasion by activating several si
Presently, there is growing interest in long-chain polyunsaturated fatty acids (LCPUFAs), which are considered a major determinant of growth, visual and neural
Background Studies showed that propentofylline enhances the action of adenosine and protects hippocampal neuronal demage against transient global cerebral ischa
Multiple endocrine neoplasia type 2 (MEN 2) is a hereditary syndrome which can present itself either in a familial form, characterized by a dominant pattern of
Background Spleen in Traditional Chinese Medicine (TCM) is not actually the spleen in the anatomic sense designated in western medicine because its functions b
Background Transforming growth factor-β1 (TGF-β1) exerts strong fibrogenic potential in culture-activated HSCs Smad 4 is a key intracellular mediator for
Chiari I malformation (CMI) is a disorder involving hindbrain maldevelopments characterized by herniation of the cerebellar tonsils through the foramen magnum.
Background This study was designed to detect methylation of E-cadherin gene promoter and gene mutation of β-catenin in exon 3 and their expression of protein
Background This study was to evaluate whether anergic cells induced by the blockade of CD40-CD154 and CD28-B7 costimulatory pathways can act as potent immunoreg