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运用ｃＤＮＡ代表性差异分析法（ｃＤＮＡｒｅｐｒｅｓｅｎｔａｔｉｏｎａｌｄｉｆｅｒｅｎｃｅａｎａｌｙｓｉｓ，ｃＤＮＡＲＤＡ），以正常人鼻咽上皮细胞及鼻咽癌ＨＮＥ１细胞作为比较的样品来源，分离了四个在鼻咽癌中缺失的ｃＤＮＡ片段．以此四个片段作探针，分别进行ＤＮＡ杂交、ＲＮＡ杂交，结果显示，这些差异性的ｃＤＮＡ序列确实来自正常人鼻咽上皮且只在其中表达和／或在鼻咽癌ＨＮＥ１中表达降低，并在鼻咽癌病人中存在不同程度的缺失．序列分析结果表明这些差异性表达的基因为具有相当抑癌基因功能的已知基因和可能与鼻咽癌相关的抑癌基因的新基因．从而说明ｃＤＮＡＲＤＡ是一种高效、敏感、假阳性低的克隆抑癌基因的有效方法
Using cDNA representative difference analysis (cDNARDA), the normal nasopharyngeal epithelial cells and nasopharyngeal carcinoma HNE1 cells were used as the source of comparative samples. Four cDNA fragments that were deleted in nasopharyngeal carcinoma were isolated. The four fragments were used as probes for DNA hybridization and RNA hybridization, respectively. The results showed that these differential cDNA sequences were indeed from the normal human nasopharyngeal epithelium and were only expressed therein and/or decreased in nasopharyngeal carcinoma HNE1. And there are different degrees of deletion in patients with nasopharyngeal carcinoma. The sequence analysis results showed that these differentially expressed genes are known genes with considerable anti-oncogene function and novel genes that may be associated with tumor suppressor genes in nasopharyngeal carcinoma. This shows that cDNARDA is an efficient, sensitive, false-positive method for cloning tumor suppressor genes