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目的研究塞来昔布在体外对人胃癌细胞BGC-823生长增殖的影响及其抗肿瘤的相关分子机制。方法体外培养人胃癌BGC-823,MTT法检测塞来昔布在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算半数抑制浓度(IC50)值;RT-PCR法检测对COX-2、MMP-9的mRNA的表达影响。结果 MTT结果显示:相同干预浓度塞来昔布抑制人胃癌细胞增殖,其24h、48h、72h的IC50分别为:158.98μmol/L、75.45μmol/L、45.08μmol/L;RT-PCR结果显示:人胃癌细胞BxPC-3正常对照组及塞来昔布干预组COX-2 mRNA灰度值分别为:0.873±0.026,0.115±0.008,P<0.05;MMP-9 mRNA灰度值分别为:0.890±0.011,0.209±0.012,P<0.05。结论塞来昔布抑制胃癌细胞增殖,塞来昔布抗肿瘤机制可能通过抑制COX-2及MMP-9的mRNA的表达来实现的。
Objective To study the effect of celecoxib on the growth and proliferation of human gastric cancer cell line BGC-823 in vitro and the related molecular mechanisms of its antitumor activity. Methods Human gastric cancer cell line BGC-823 was cultured in vitro. The effect of celecoxib on the proliferation of gastric cancer cells at different concentrations was tested by MTT assay. The half-value inhibition concentration (IC50) MMP-9 mRNA expression. Results MTT results showed that IC50 of celecoxib at the same concentration for 24 h, 48 h and 72 h were 158.98μmol / L, 75.45μmol / L and 45.08μmol / L, respectively. The results of RT-PCR showed that: The gray value of COX-2 mRNA in human gastric cancer cell BxPC-3 normal control group and celecoxib intervention group were 0.873 ± 0.026 and 0.115 ± 0.008 respectively, P <0.05; the gray value of MMP-9 mRNA were 0.890 ± 0.011, 0.209 ± 0.012, P <0.05. Conclusion Celecoxib inhibits the proliferation of gastric cancer cells. The anti-tumor mechanism of celecoxib may be through inhibiting the mRNA expression of COX-2 and MMP-9.