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目的 研究抑癌基因Rb在人胶质瘤SHG 44细胞调亡前后的表达情况。方法 利用N端Rb蛋白单克隆抗体通过Western印迹法检测SHG 44胶质瘤细胞株Rb蛋白的表达情况 ,并观察用VP16诱导细胞凋亡后Rb蛋白表达的改变。结果 在SHG 44胶质瘤细胞株中存在一相对分子质量约 70× 10 3 、具有N端的Rb蛋白。细胞凋亡后 ,Rb蛋白有裂解现象 ,出现一具有N端的约 48× 10 3 的裂解蛋白 ,并使细胞停滞于G1期。结论 在胶质瘤SHG 44细胞中有Rb基因突变体的表达 ,Rb蛋白的裂解与细胞凋亡密切相关。
Objective To study the expression of tumor suppressor gene Rb before and after apoptosis of human glioma SHG 44 cells. Methods The expression of Rb protein in SHG 44 glioma cell line was detected by Western blotting using N-terminal Rb protein monoclonal antibody and the change of Rb protein expression was observed after induced by VP16. Results There was an Rb protein with a molecular weight of about 70 × 10 3 in the SHG 44 glioma cell line. After apoptosis, the Rb protein is cleaved, presenting an approximately 48 × 10 3 cleavage protein with an N-terminus and arresting the cells in the G1 phase. Conclusion The expression of Rb gene mutant in SHG 44 glioma cells is closely related to the apoptosis of Rb protein.