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用降植烷、石蜡油、不完全佐剂(以下称三种预处理药物)分别诱导小鼠产生腹水型MCAb。研究了三种预处理药物的致敏剂量,致敏后与接种杂交瘤的间隔时间、杂交瘤细胞接种数量,腹水产量,抗体效价和小鼠成活期等。实验结果表明三种预处理药物中的任何一种
Mice were induced to produce ascitic MCAb by using pristane, paraffin oil and incomplete adjuvant (hereinafter referred to as three pretreatment drugs) respectively. The sensitizing doses of three pretreatment drugs, the interval between sensitized and inoculated hybridomas, the number of inoculated hybridoma cells, the yield of ascites, antibody titer, and mouse survival were studied. The experimental results show any of the three pretreatment drugs