论文部分内容阅读
采用琼脂糖电泳法观察５种抗癌药对白血病细胞ＤＮＡ的嵌合能力。结果发现表阿霉素同进口阿霉素在５０μｇ／ｍｌ浓度时可与ＤＮＡ发生嵌合效应；国产阿霉素在较高浓度（１００μｇ／ｍｌ）和柔红霉素需高浓度（２００μｇ／ｍｌ）才显示嵌合效应；而米托蒽醌在较低浓度（２５μｇ／ｍｌ）即可发生此作用。本实验体现了作用机制类同的抗肿瘤药物对ＤＮＡ嵌合能力的差异。
Agarose gel electrophoresis was used to observe the chimerism of five anticancer drugs on DNA of leukemia cells. The results showed that epirubicin and adriamycin at the concentration of 50μg / ml chimerism can occur with DNA; domestic doxorubicin at higher concentrations (100μg / ml) and daunorubicin need high concentration (200μg / ml ) Showed chimerism, whereas mitoxantrone took place at a lower concentration (25 μg / ml). This experiment reflects the same mechanism of action of anticancer drugs on DNA chimerism differences.