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本试验目的是检验在胰岛素靶组织中Leptin是否对葡萄糖转运有直接作用。设计与方法 采用两个试验方案:(1)骨骼肌试验,SD鼠磨碎后取肱骨内上踝肌及另外鼠比目鱼肌。(2)脂肪细胞,用CO2吸入或颈脱位法杀死鼠后剥离外部脂肪垫。观察肌肉或脂肪细胞在无或有重
The purpose of this experiment was to examine whether Leptin has a direct effect on glucose transport in insulin target tissues. DESIGN AND METHODS Two experimental protocols were used: (1) Skeletal muscle test: the upper ankle muscles of the humerus and the soleus muscles of the other rats were harvested from the SD rats. (2) Adipocytes, stripped of external fat pad by CO2 inhalation or cervical dislocation. Muscle or fat cells observed in the absence or weight