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目的 观察低氧预处理对大鼠海马神经元缺氧耐受性和IL 6免疫反应的影响。方法 取培养 12d的两组 (对照组和低氧预处理组 )培养神经元 ,同时置于缺氧环境 ( 0 .9L LN2 、0 .1L LCO2 )中培养 2、4、8和 12h。分别观察它们的形态变化和神经元存活数 ,并用抗rhIL 6单克隆抗体进行免疫组化染色 ,观察缺氧对大鼠海马培养神经元IL 6免疫反应的影响。结果 低氧预处理可增强海马神经元对rhIL 6的免疫反应 ,经低氧预处理的海马神经元缺氧后神经元存活数和对rhIL 6的免疫反应均明显高于对照组。结论 低氧预处理可使体外培养的海马神经元对缺氧产生耐受 ,其中rhIL 6的免疫反应增加可能是海马神经元对缺氧的一种适应性变化 ,提示IL 6可能参与脑缺氧耐受性的形成 ,并在海马神经元缺氧损伤的调控中起重要作用。
Objective To observe the effects of hypoxic preconditioning on hypoxic tolerance and IL 6 immunoreactivity in hippocampal neurons of rats. Methods The neurons were cultured in two groups (control group and hypoxic preconditioning group) cultured for 12 days and cultured in hypoxic environment (0.9L LN2, 0.1L LCO2) for 2, 4, 8 and 12 hours. Morphological changes and neuron survival numbers were observed respectively. Immunohistochemical staining was performed with anti-rhIL 6 monoclonal antibody to observe the effect of hypoxia on IL 6 immunoreactivity in cultured hippocampal neurons. Results Hypoxic preconditioning increased the immunological reaction of hippocampal neurons against rhIL 6. The neuronal survival rate and the immune response to rhIL 6 in hypoxic preconditioned hippocampal neurons were significantly higher than those in control group. CONCLUSION: Hypoxic preconditioning can make hippocampal neurons cultured in vitro tolerant to hypoxia. The increased immune response of rhIL 6 may be an adaptive change of hippocampal neurons to hypoxia, suggesting that IL 6 may be involved in cerebral hypoxia Tolerance formation and play an important role in the regulation of hypoxic injury in hippocampal neurons.