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目的:研究15株多重耐药金黄色葡萄球菌耐药基因和肠毒素基因定位特征。方法:对15株多重耐药金黄色葡萄球菌质粒消除前后,进行药敏试验和肠毒素测定比较,从而对耐药基因和肠毒素基因进行定位。结果:15株多重耐药金黄色葡萄球菌耐药基因大多数位于质粒上,由质粒介导的耐药基因占70.6%,由染色体编码的耐药基因占29.4%。而对于肠毒素基因,发现SEA、SEC位于染色体上,SED位于质粒上,而SEB有位于染色体或质粒上。结论:耐药基因、肠毒素基因可以定位到染色体或质粒上,且质粒在决定耐药性中起主要作用。
Objective: To study the multidrug-resistant Staphylococcus aureus drug resistance genes and enterotoxin gene mapping characteristics. Methods: Before and after elimination of 15 multidrug-resistant Staphylococcus aureus plasmids, antibiotic susceptibility test and enterotoxin test were performed to determine the resistance genes and enterotoxin genes. Results: Most of the 15 multidrug-resistant Staphylococcus aureus genes were located on the plasmid, 70.6% were plasmid-mediated and 29.4% were gene-coded by chromosome. For enterotoxin genes, SEA was found, with the SEC on the chromosome, the SED on the plasmid, and the SEB on the chromosome or plasmid. Conclusion: The resistance genes and enterotoxin genes can be mapped to chromosomes or plasmids, and the plasmids play a major role in determining drug resistance.