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利用壳聚糖/纳米金/3-氨丙基三乙烯硅烷/普鲁士蓝复合膜将Hb固载于玻碳电极上制成Hb电化学传感器,研究了Hb在该传感器上的直接电化学行为。分别用紫外-可见光谱、红外光谱、循环伏安法对复合膜进行了表征,结果表明,该修饰膜为Hb提供了生物相容的微环境,并有效地促进了Hb的电子传递。Hb在复合膜中的式电位为2.05×10-1V,Hb Fe(Ⅲ)/Fe(Ⅱ)电对的式量电位与溶液p H值呈线性关系,表明Hb在该修饰电极上的电化学为一个质子伴随一个电子的电极过程。该修饰电极对H2O2具有良好的催化作用,峰电流与H2O2浓度在2.00×10-6~4.80×10-4mol/L范围内呈良好线性关系,检测限为1.00×10-7mol/L(S/N=3),表观米氏常数KMapp为1.99 mmol/L。该传感器具有响应快、灵敏度高及稳定性好等优点。
Hb electrochemical sensor was fabricated by immobilizing Hb on glassy carbon electrode with chitosan / nano-gold / 3-aminopropyltriethoxysilane / Prussian blue composite membrane. The direct electrochemical behavior of Hb on this sensor was studied. The composite films were characterized by UV-Vis, FTIR and cyclic voltammetry respectively. The results showed that the modified films provided a biocompatible microenvironment for Hb and effectively promoted the electron transfer of Hb. The potential of Hb in the composite membrane is 2.05 × 10-1V. The linear relationship between the mass potential of Hb Fe (Ⅲ) / Fe (Ⅱ) ion pair and the p H value of the solution indicates that the electrochemical performance of Hb on the modified electrode A proton accompanies an electronic electrode process. The modified electrode has a good catalytic effect on H2O2. The peak current has a good linear relationship with the concentration of H2O2 in the range of 2.00 × 10-6 ~ 4.80 × 10-4 mol / L with the detection limit of 1.00 × 10-7mol / L (S / N = 3) and the apparent Michaelis constant KMapp is 1.99 mmol / L. The sensor has the advantages of fast response, high sensitivity and good stability.