采用纳米普鲁士蓝/金纳米粒子/壳聚糖(nano-PB/AuNPs/Chit)复合膜固定葡萄糖氧化酶(GOD)构建新型葡萄糖生物传感器。通过电化学阻抗谱以及电流-时间曲线法(I-t)研究了传感器的电化学特性。结果表明,传感器在葡萄糖浓度为0.01～1.0 mmol/L范围内呈线性,响应灵敏度为68.15μA.(mmol/L)-1.cm-2,表观米氏常数为5.1 mmol/L。该传感器可用于糖尿病人血糖的测定。 A novel glucose biosensor was constructed by immobilizing glucose oxidase (GOD) with nanosized Prussian blue / gold nanoparticle / chitosan (nano-PB / AuNPs / Chit) composite membrane. The electrochemical properties of the sensor were investigated by electrochemical impedance spectroscopy and current-time curve method (I-t). The results showed that the sensor was linear in the range of 0.01-1.0 mmol / L glucose with a response sensitivity of 68.15 μA (mmol / L) -1.cm-2 and an apparent Michaelis constant of 5.1 mmol / L. The sensor can be used for the determination of blood glucose in diabetic patients.