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分别用两种方法研究了人原发性肝细胞癌总基因组及特定癌基因片段的DNA甲基化情况，并进行对比研究。以3H－SAM掺入法研究总基因组DNA甲基化水平，以限制性内切酶酶切、South－ern吸印法对c－myc、c－N－ras两种癌基因状况进行分析。结果：发现人肝细胞癌区和癌旁区组织内c－myc、c－N－ras癌基因分别有不同程度的低甲基化，且有癌区总基因DNA甲基化水平明显下降。本文结果提示：两种方法检测均发现在人原发性肝癌中存在DNA低甲基化现象，联用两种方法效果优于单一检测者。
Two methods were used to study the DNA methylation status of human primary hepatocellular carcinoma and specific oncogene fragments, and a comparative study was conducted. 3H-SAM incorporation was used to study the level of total genomic DNA methylation, and the status of c-myc and c-N-ras oncogenes was analyzed by restriction endonuclease digestion and South-ern blotting. RESULTS: It was found that the c-myc and c-N-ras oncogenes in human hepatocellular carcinoma and paracancerous tissues were hypomethylated to varying degrees, and the total gene DNA methylation levels in the cancer regions were significantly decreased. The results of this study suggest that both methods have detected hypomethylation of DNA in human primary liver cancer, and the combination of the two methods is superior to a single tester.