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发展了研究脱落酸（ＡＢＡ）结合蛋白的两类免疫探针。其一，用ＡＢＡ－Ｃ１－ＢＳＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱纯化出ＡＢＡ结合蛋白，聚丙烯酰胺凝胶电泳显示该蛋白分子量为５６ＫＤ的一条带，它具有特异结合ＡＢＡ的能力（Ｋｄ＝２．０×１０－９ｍｏｌ／Ｌ）。当用蛋白水解酶Ｋ水解该蛋白，并用１％琼脂糖凝胶电泳时，发现其中存在约３００个核苷酸的ｒＲＮＡ分子。用识别ＡＢＡ结合蛋白的抗体筛选ｃＤＮＡ表达文库，从２００，０００个独立噬斑中获得１２０个编码玉米１７ｓＲＮＡ的ｃＤＮＡ克隆和１个ｃＤＮＡ编码结合蛋白的ｃＤＮＡ克隆（２４ｃＤＮＡ）。２４ｃＤＮＡ有１０７５个碱基对，含编码２５４个氨基酸的开放阅读框架。其二，制备了识别抗ＡＢＡ单克隆抗体的独特型抗体（ａｎｔｉ－Ｉｄ），它具有模拟并竞争ＡＢＡ的能力。用上述两类免疫探针定位了植物细胞中的ＡＢＡ结合蛋白。发展了研究脱落酸（ＡＢＡ）结合蛋白的两类免疫探针。其一，用ＡＢＡ－Ｃ１－ＢＳＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱纯化出ＡＢＡ结合蛋白，聚丙烯酰胺凝胶电泳显示该蛋白分子量为５６ＫＤ的一条带，它具有特异结合ＡＢＡ的能力（Ｋｄ＝２．０×１０－９ｍｏｌ／Ｌ）。当用蛋白水解酶Ｋ水解该蛋?
Two types of immuno probes have been developed to study abscisic acid (ABA) binding proteins. First, ABA-binding protein was purified by ABA-C1-BSA-Sepharose 4B affinity chromatography. Polyacrylamide gel electrophoresis showed a 56KD band with the ability to specifically bind ABA (Kd = 2). 0 × 10 -9 mol / L). When this protein was hydrolyzed with proteolytic enzyme K and electrophoresed on a 1% agarose gel, a rRNA molecule of about 300 nucleotides was found therein. The cDNA expression library was screened with an antibody that recognizes the ABA binding protein and 120 cDNA clones encoding corn 17sRNA and 1 cDNA cDNA encoding the binding protein (24 cDNA) were obtained from 200,000 independent plaques. The 24 cDNA has 1075 base pairs and contains an open reading frame of 254 amino acids. Second, anti-Id antibodies recognizing anti-ABA monoclonal antibodies were prepared that have the ability to mimic and compete for ABA. ABA-binding proteins in plant cells were located using the above two types of immunoprobes. Two types of immuno probes have been developed to study abscisic acid (ABA) binding proteins. First, ABA-binding protein was purified by ABA-C1-BSA-Sepharose 4B affinity chromatography. Polyacrylamide gel electrophoresis showed a 56KD band with the ability to specifically bind ABA (Kd = 2). 0 × 10 -9 mol / L). When using the proteolytic enzyme K hydrolysis of the egg?