目的　探讨新克隆的CKIp15 INK4B 相关基因p15rs对细胞增殖的作用。　方法　克隆p15rs编码区cDNA ,通过基因转染技术将其导入HeLa细胞 ,检测细胞的生长曲线 ,细胞周期和集落形成能力。　结果　构建了p15rs高表达的细胞模型HPS2 1,发现p15rs高表达可使HeLa细胞增殖被显著抑制 ,细胞生长速率下降 ,倍增时间延长 ,G1 期细胞增加 ,S期细胞减少 ,集落形成能力下降。　结论　新基因p15rs具有抑制癌细胞增殖和对细胞周期进程进行负调节的作用。 Objective To investigate the effect of newly cloned p15rs gene of CKIp15 INK4B on cell proliferation. Methods The cDNA of p15rs was cloned and transfected into HeLa cells by gene transfection. The cell growth curve, cell cycle and colony formation ability were detected. Results High expression of p15rs cell line HPS2 1 was constructed. It was found that high expression of p15rs could significantly inhibit HeLa cell proliferation, decrease cell growth rate, prolong doubling time, increase cells in G1 phase, decrease cells in S phase and decrease colony forming ability. Conclusion The new gene p15rs inhibits the proliferation of cancer cells and negatively regulates the cell cycle progression.