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为研究低分子柑橘果胶的抗前列腺癌细胞活性,以商品化柑橘果胶为原料,121℃处理30min制得低分子柑橘果胶,测定了其分子质量、半乳糖醛酸含量、酯化度和体外抗人前列腺癌激素不依赖型PC3细胞活性。结果表明,制得的低分子柑橘果胶的平均分子质量为6685u、半乳糖醛酸含量为78%、酯化度为38%,柑橘果胶中对应的数值分别为55654u、64%、59%。低分子柑橘果胶对PC3细胞有很强的抑制作用,中剂量(2.5mg/mL)下抑制率可达38%,高剂量(5～10mg/mL)下抑制率达80%～90%,肿瘤细胞增殖(72h)的半数抑制浓度为(3.55±0.44)mg/mL,相对于天然的柑橘果胶,其抗前列腺癌细胞活性显著增强。
In order to study the anti-prostate cancer cell activity of low-molecular citrus pectin, commercial citrus pectin was used as raw material and treated at 121 ℃ for 30min to prepare low molecular citrus pectin. The molecular weight, galacturonic acid content, degree of esterification And in vitro anti-human prostate cancer hormone-independent PC3 cell activity. The results showed that the average molecular weight of the obtained low-molecular citrus pectin was 6685u, the content of galacturonic acid was 78%, the degree of esterification was 38%, the corresponding values of citrus pectin were 55654u, 64%, 59% . Low-molecular citrus pectin had a strong inhibitory effect on PC3 cells, the inhibition rate was 38% at medium dose (2.5mg / mL) and 80% -90% at high dose (5-10mg / mL) The median inhibitory concentration (3.55 ± 0.44) mg / mL for tumor cell proliferation (72h) was significantly higher than that of natural citrus pectin.