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[目的]研究黄连素对人肝癌HepG2细胞增殖和血管内皮细胞生长因子(vascular endothelial growth fac-tor,VEGF)表达的影响。[方法]分别以不同浓度(10、25、50、100μmol/L)的黄连素作用HepG2细胞24h后,采用MTT法观察细胞增殖变化、实时定量PCR(Real-time PCR)检测VEGF mRNA表达水平变化、Western blot检测VEGF蛋白水平变化。[结果]黄连素可抑制HepG2细胞的增殖,并呈浓度依赖性;不同浓度的黄连素作用细胞24h后,HepG2细胞VEGF mRNA和蛋白表达均有所下降,且呈现出一定的量效关系。[结论]黄连素可抑制人肝癌HepG2细胞的增殖和VEGF的表达,抗血管生成可能是黄连素抗肝肿瘤的作用机制之一。
[Objective] To investigate the effect of berberine on the proliferation of HepG2 cells and the expression of vascular endothelial growth fac-tor (VEGF). [Methods] HepG2 cells were treated with berberine at different concentrations (10, 25, 50 and 100 μmol / L) for 24 h respectively. MTT assay was used to observe the changes of cell proliferation. Real-time PCR was used to detect the expression of VEGF mRNA , Western blot detection of VEGF protein levels. [Result] Berberine could inhibit the proliferation of HepG2 cells in a concentration-dependent manner. The expression of VEGF mRNA and protein of HepG2 cells decreased with different concentrations of berberine for 24 hours, and showed a dose-effect relationship. [Conclusion] Berberine inhibits the proliferation and the expression of VEGF in HepG2 human hepatocellular carcinoma. Antiangiogenesis may be one of the mechanisms of berberine in inhibiting liver tumors.