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目的:建立烧伤擦剂的质量标准。方法:采用TLC法对方中黄连、黄柏进行定性鉴别,采用HPLC法测定制剂中盐酸小檗碱的含量。结果:薄层色谱斑点清晰,分离度好;盐酸小檗碱在0.2～1.2μg范围内,与峰面积呈良好线性关系(r=0.9997),平均回收率为99.07%,RSD=0.31%(n=6)。结论:该方法专属性强,准确,重复性好,简便易行,可作为烧伤擦剂的质量控制方法。
Objective: To establish the quality standard of burn liniment. Methods: The contents of berberine hydrochloride in the preparations were determined by TLC. Results: The TLC spots were clear and the resolution was good. Berberine hydrochloride showed a good linear relationship with the peak area (r = 0.9997) in the range of 0.2-1.2 μg with an average recovery of 99.07% and a RSD of 0.31% (n = 6). Conclusion: The method is specific, accurate, reproducible and simple, and can be used as a quality control method for burn liniment.