论文部分内容阅读
目的:本实验采用唐传(厦门)生物科技有限公司提供的L-阿拉伯糖对喂养高糖高脂肪饲料的新西兰大白兔空腹血糖和糖耐量作用的影响进行研究,为将L-阿拉伯糖应用到预防和辅助治疗2型糖尿病以及防止高糖高脂饮食引起的糖代谢紊乱提供科学数据。方法:选择体重在1.8～2.0kg范围新西兰大白兔32只,随机分为A,B,C,D维,每组8只,其中A～C组均喂养高糖高脂和高胆固醇饲料,D组喂普通饲料,同时采用软滴管代替以往使用的导胃管的方法,A组口服1.24g/d,B组口服1.11g/d L-阿拉伯糖进行干预。分别于30天,60天检测空腹血糖,糖耐量。结果:本实验采用软滴管进行兔子定量口服喂食液体的方法证明,该方法比以往的导胃管方法方便,实施过程对兔子安全,而且在喂的过程中对兔子的刺激少,减少因为过分刺激对兔子的生理状态产生的影响。30天的检测结果证明,A,B,C,D组兔子间检测指标差异无显著性;60天后检测结果证明,A,B组空腹血糖比C组低,有显著性差异(P<0.05);糖耐量AUC值A,B组比C组明显小,差异显著(P<0.05)。结论:为今后兔子定量口服液体食物提供一个科学有效的方法,并且证明L-阿拉伯糖对喂养高糖高脂肪食物的兔子防止高血糖以及增强糖耐量作用方面效果明显,为L-阿拉伯糖应用于预防糖尿病防止因为平常高糖高脂肪饮食引起的糖尿病提供科学依据。
Aims: To investigate the effect of L-arabinose supplied by Tang Chuan (Xiamen) Biotechnology Co., Ltd. on fasting blood glucose and glucose tolerance in New Zealand white rabbits fed with high-sugar and high-fat diets. In order to apply L-arabinose Provide scientific data for prevention and adjuvant treatment of type 2 diabetes and prevention of disorders of glucose metabolism caused by high-sugar and high-fat diet. Methods: Thirty-two New Zealand white rabbits weighing 1.8-2.0 kg were randomly divided into A, B, C, and D groups, with 8 rats in each group. All rats in groups A to C were fed with high-fat and high- Group fed normal feed, while the use of soft tube to replace the past use of the method of gastric tube, A group oral 1.24g / d, B group oral 1.11g / d L-arabinose intervention. Fasting blood glucose and glucose tolerance were measured at 30 days and 60 days respectively. Results: The method of using soft dropper to quantitatively orally feed rabbit serum proved that this method is more convenient than the traditional method of guiding the stomach and the rabbit is safe in the process of implementation, less stimulation to rabbits in the process of feeding, Stimulation of the rabbit’s physiological state of the impact. The results of 30 days showed that there was no significant difference between the A, B, C and D groups. The results of the test after 60 days showed that the fasting blood glucose in group A and group B was lower than that in group C (P <0.05) ; AUC of AUC in A and B groups were significantly smaller than that in C group (P <0.05). Conclusion: This study provides a scientific and effective method for the quantitative oral administration of liquid food in rabbits in the future and proves that L-arabinose can effectively prevent hyperglycemia and enhance glucose tolerance in rabbits fed with high-sugar and high-fat foods. Prevention of diabetes prevents diabetes because of the usual high-sugar and high-fat diet to provide a scientific basis.