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目的研制产朊假丝酵母尿酸酶纳米脂质体(nanoliposomes containing uricase from candida utilis,UCCNLs)并对其理化性质和体外活性进行评价。方法采用逆向蒸发法制备UCCNLs,以包封率、最小多分散指数、粒径等为评价指标优化制备工艺。考察体外降尿酸活性。考察4,25℃纳米粒的稳定性。结果醚水比为3∶1,脂醇比为1∶1,投药量为2 mg时,UCCNLs的平均包封率为64.24%,平均粒径为206.73 nm,多分散系数为0.247,Zeta电位为-37.33 mV。将尿酸从0.595 mol.L-1降至正常水平UCCNLs所需时间约为游离酶的一半。UCCNLs在4℃时具有良好的稳定性。结论采用逆向蒸发法可制备UCCNLs,工艺简便,包封率高,稳定性较好,体外降尿酸效果好。
OBJECTIVE: To develop the nanoliposomes containing uricase from candida utilis (UCCNLs) and evaluate their physico-chemical properties and in vitro activity. Methods UCCNLs were prepared by reverse evaporation. The encapsulation efficiency, minimum polydispersity index and particle size were used as evaluation indexes to optimize the preparation process. In vitro study uric acid activity. Investigate the stability of 4,25 ℃ nanoparticles. The results showed that the average entrapment efficiency of UCCNLs was 64.24%, the average particle size was 206.73 nm, the polydispersity coefficient was 0.247 and the Zeta potential was -37.33 mV. The time it takes to reduce uric acid from 0.595 mol.L-1 to normal levels of UCCNLs is about half that of free enzymes. UCCNLs have good stability at 4 ° C. Conclusion The reverse evaporation method can be used to prepare UCCNLs, which has the advantages of simple process, high entrapment efficiency, good stability and good uric acid reduction in vitro.