论文部分内容阅读
取番茄不同苗龄的无菌苗子叶进行培养，发现以１４天苗龄的子叶培养不定芽再生频率最高，在ＭＳ培养基上添加不同浓度的吲哚乙酸（ＩＡＡ）和玉米素（Ｚｔ），对子叶愈伤组织的形成影响不大，但对不定芽的分化有较大影响，其中以ＭＳ＋Ｚｔ１ｍｇ·Ｌ~（－１）＋ＩＡＡ１ｍｇ·Ｌ~（－１）效果最好。在同样的培养基上继代培养，待芽长至１～２ｃｍ长时转到生根培养基上即可在短期内长出根系，成为再生植株。
Aseptic shoots of tomato seedlings were cultured at different seedling ages. It was found that the regeneration frequency of adventitious buds was the highest in cotyledons of 14-day-old seedlings. Different concentrations of indole acetic acid (IAA) and zeatin (Zt) were added to MS medium, There was little effect on the formation of cotyledon callus, but had a significant effect on the differentiation of adventitious buds. MS + Zt1mg · L -1 + IAA1mg · L -1 was the best. In the same medium subculture, until the buds grow to 1 ~ 2cm long when transferred to the rooting medium can grow in the short term root system, a regenerated plant.