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目的 :建立恶性肿瘤细胞经血循环向骨骼肌运行的动物模型 ,观察骨骼肌转移瘤罕见性现象 ,并进一步探讨骨骼肌微血管内皮细胞粘附分子 ( VCAM- 1)的意义。方法 :Wistar大鼠 60只 ,随机分成实验组与对照组。实验组经髂动脉注入 Walker2 5 6癌肉瘤单细胞悬液 ( 10 4) ,对照组经尾静脉注入同一瘤细胞 ( 10 6) ,两组分别于 2h、 1d、 7d、 14 d各处死 6只 ,余下 6只自然死亡 ,观察比较肺内、大腿骨骼肌内肿瘤形成情况及微血管内皮细胞VCAM- 1表达情况。结果 :实验组注入瘤细胞后 ,第 7天在大腿骨骼肌微血管周围见瘤细胞团 ( 3只 ) ,肌组织内无转移灶形成 ,部分鼠见骨骼肌旁软组织内有转移灶形成 ;对照组注入瘤细胞后肺内 7d形成肿瘤灶 ,14 d及自然死亡者 10 0 %形成广泛肺转移灶 (共 17只 ) ,两组比较差异有显著意义 ( P<0 .0 0 1)。实验组大腿骨骼肌微血管内皮VCAM- 1阳性率在注瘤 d7以后显著上升 ,且实验组大腿骨骼肌与对照组肺微血管内皮细胞 VCAM- 1阳性率无显著差异 ( P=0 .5 )。结论 :临床上广泛存在的恶性肿瘤骨骼肌转移罕见性 ,可在动物模型中观察到。骨骼肌微血管内皮细胞 VCAM- 1的表达 ,不能解释骨骼肌转移瘤罕见性
OBJECTIVE: To establish an animal model of malignant tumor cells running to the skeletal muscle by blood circulation and to observe the rare phenomenon of skeletal muscle metastases, and to further explore the significance of skeletal muscle microvascular endothelial cell adhesion molecule (VCAM-1). Methods: Sixty Wistar rats were randomly divided into experimental group and control group. The experimental group was infused with single cell suspension of Walker2 5 6 carcinosarcoma (104) via the iliac artery, and the control group was injected with the same tumor cell via the tail vein (106). The two groups were sacrificed at 2h, 1d, 7d and 14d , And the remaining 6 died of natural death. The formation of tumors in the lung and thigh skeletal muscle and the expression of VCAM-1 in microvascular endothelial cells were observed and compared. Results: After injection of tumor cells into the experimental group, on the 7th day, tumor cell clusters (3) were seen around the capillaries of the thigh muscles. There was no metastasis in the muscle tissue and some mice showed metastases in the soft tissue around the skeletal muscle. In the control group After tumor cells were injected into the lung for 7 days, tumors formed on the 7th day and 10% of the natural deaths formed extensive lung metastases (17 cases in total). There was significant difference between the two groups (P <0.01). The positive rate of VCAM-1 in the thoracic muscle microvascular endothelium of experimental group increased significantly after d7, and there was no significant difference in the positive rate of VCAM-1 between the experimental group and the control group (P = 0.5). CONCLUSIONS: The clinically widespread skeletal muscle metastases of malignant tumors are rare and can be observed in animal models. Skeletal muscle microvascular endothelial cells VCAM-1 expression, can not explain the rare skeletal muscle metastases