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目的探讨原花青素(OPC)对C57BL/6J小鼠血管内皮舒张功能的影响及物质基础。方法①将内皮功能紊乱的C57BL/6J小鼠分为对照组、0.1%w/w原花青素组、抗生素组、抗生素+0.1%w/w原花青素组,干预4周后观察小鼠胸主动脉舒张功能;②采用高效液相色谱法(HPLC)测定OPC(100mg/kg)灌胃后小鼠60min血浆中OPC以及其代谢物原儿茶酸(PCA)含量;③将内皮功能紊乱的C57BL/6J小鼠分为抗生素组、抗生素+0.1%w/wOPC、抗生素+0.1%w/wOPC+0.003%w/wPCA,干预4周后观察小鼠胸主动脉舒张功能。结果①OPC干预可提高小鼠动脉舒张功能,但该效应被抗生素阻断;②OPC灌胃小鼠60min后,HPLC法测得血浆PCA浓度为(10.6±1.8)μmol/L,但OPC未检出;③抗生素阻断OPC介导的小鼠动脉舒张功能可被其代谢物PCA部分恢复。结论原花青素可以改善血管内皮舒张功能,其物质基础可能与其代谢物PCA有关。
Objective To investigate the effects of proanthocyanidins (OPCs) on vascular endothelial relaxation in C57BL / 6J mice and its material basis. Methods ① C57BL / 6J mice with endothelial dysfunction were divided into control group, 0.1% w / w proanthocyanidin group, antibiotic group and 0.1% w / w proanthocyanidin group. After 4 weeks of intervention, the diastolic function of thoracic aorta ; ② The content of OPC and its metabolite protocatechuic acid (PCA) in the plasma of the mice after 60 min of OPC (100 mg / kg) administration was determined by high performance liquid chromatography; ③ The endothelial dysfunction C57BL / 6J The rats were divided into antibiotic group, antibiotic + 0.1% w / w OPC, antibiotic + 0.1% w / w OPC plus 0.003% w / w PCA, and the thoracic aorta diastolic function was observed after 4 weeks intervention. Results ① The intervention of OPC increased diastolic arterial function in mice, but the effect was blocked by antibiotics. ② The concentration of PCA in plasma was (10.6 ± 1.8) μmol / L, ③ Antibiotics blocked OPC-mediated mouse diastolic function can be part of its metabolites PCA recovery. Conclusion Proanthocyanidins can improve the vascular endothelial relaxation function, and its material basis may be related to its metabolites PCA.