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目的:探讨miR-125b对宫颈癌细胞放射敏感性的影响及其可能的下游机制研究。方法:实时荧光定量聚合酶链反应(quantitative real-time PCR，RT-qPCR)检测宫颈癌组织和细胞中miR-125b和Foxp3的表达量。HeLa细胞进行梯度剂量X射线(0、2、4、6 Gy)照射后，RT-qPCR检测HeLa细胞中miR-125b和Foxp3的表达量。下调miR-125b表达，经6 Gy X射线照射，MTT试验检测HeLa细胞增殖能力，Western blot检测Bax和Bcl-2蛋白表达量。通过Targetscan和双荧光素报告基因实验检测miR-125b与Foxp3的靶向关系。下调Foxp3表达，经6 Gy X射线照射，MTT试验检测HeLa细胞增殖能力，Western blot检测Bax和Bcl-2蛋白表达量。检测miR-125b通过Foxp3对HeLa细胞放射敏感性的影响。下调Foxp3后通过ELISA检测细胞上清液中IL-10和TGF-β的含量。结果:宫颈癌组织和细胞中miR-125b的表达量显著降低，Foxp3表达量显著升高，miR-125b的表达量随着X射线放射剂量增加而升高，Foxp3表达量随着X射线放射剂量增加而降低。6 Gy X射线照射HeLa细胞后，下调miR-125b提高细胞增殖能力，使Bax表达量明显降低，Bcl-2表达量明显升高。miR-125b靶向Foxp3且负调控Foxp3表达。6 Gy X射线照射细胞后，下调Foxp3可显著降低HeLa细胞增殖能力，使Bax表达量明显升高，Bcl-2表达量明显降低。过表达miR-125b能够通过Foxp3增强HeLa细胞的放射敏感性。6 Gy X射线照射细胞后，下调Foxp3降低了细胞中IL-10和TGF-β的表达。结论:上调miR-125b通过靶向和负调控Foxp3，从而增强宫颈癌细胞的放射敏感性，且作用机制可能与下调Foxp3使细胞中IL-10和TGF-β的表达减少有关。“,”Objective:To investigate the effects of miR-125b on radiosensitivity of cervical cancer cells and its possible downstream mechanism.Methods:The expression of miR-125b and Foxp3 in cervical cancer tissues and cells was detected by RT-qPCR. The HeLa cells were irradiated with 0, 2, 4 and 6 Gy of X-rays. The expression of miR-125b and Foxp3 in each group was detected by RT-qPCR. After downregulation of miR-125b expression and 6 Gy X-ray irradiation, the proliferation ability of HeLa cells was detected by MTT assay, and the expression of Bax and Bcl-2 proteins were detected by Western blot. The relationship between miR-125b and Foxp3 was detected by Targetscan and Dual luciferin reporter assay. After downregulation of Foxp3 expression and 6 Gy X-ray irradiation, the proliferation ability of HeLa cells was detected by MTT assay, and the expression of Bax and Bcl-2 proteins were detected by Western blot. The effects of miR-125b on radiosensitivity of HeLa cells through Foxp3 were detected. After down-regulation of Foxp3, the contents of IL-10 and TGF-β in supernatant were detected by ELISA.Results:The expression of miR-125b in the tissues and cells of cervical cancer was significantly decreased, while the expression of Foxp3 was significantly increased. The expression of miR-125b in HeLa cells was increased after radiation in a dose dependent manner. The expression of Foxp3 in HeLa cells was decreased after radiation in a dose dependent manner. After 6 Gy X-ray irradiation of HeLa cells, down-regulation of miR-125b increased the cell proliferation capacity, significantly reduced the expression of Bax and increased the expression of Bcl-2. miR-125b targets Foxp3 and negatively regulates Foxp3 expression. After 6 Gy X-ray irradiation of HeLa cells, down-regulation of Foxp3 significantly reduced the proliferation capacity of HeLa cells, increased the expression of Bax and decreased the expression of Bcl-2. Overexpression of miR-125b can enhance radiosensitivity of HeLa cells through Foxp3.After 6 Gy X-ray irradiation, down-regulation of Foxp3 reduced the expression of IL-10 and TGF-β in cells.Conclusions:Upregulation of miR-125b enhances the radiosensitivity of cervical cancer cells by targeting and negatively regulating Foxp3, and the mechanism of that may be related to the down-regulation of Foxp3 to reduce the expression of IL-10 and TGF-β in the cells.