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作者应用肿瘤坏死因子-α(TNF-α)和淋巴细胞毒紊(LT)对7例B细胞慢性淋巴细胞白血病(BCLL)细胞增殖作用进行了研究。以5例年龄、性别配对的健康人作对照。 方法:取患者和对照者静脉血,肝素抗凝分离出单个核细胞(PBMC),用玫瑰花结法和CD_3、CD_(14)单克隆抗体加兔补体除去T细胞,即得纯化的B细胞;将纯化的B细胞5×10~4细胞/孔加入96孔板内,分别培养于完全1640培养基,含γTNF-α的完全培养基或含γ-LT的培养基中,培养3、5或7天。
The authors investigated the proliferation of 7 B-cell chronic lymphocytic leukemia (BCLL) cells using tumor necrosis factor-α (TNF-α) and lymphocyte cytotoxicity (LT). Five healthy people, matched by age and sex, were used as controls. Methods: Mononuclear cells (PBMCs) were isolated from patients and controls by venous blood and anticoagulated with heparin. T lymphocytes were removed by rosetting with CD_3 and CD_ (14) monoclonal antibodies and rabbit complement to obtain purified B cells ; Purified B cells 5 × 10 ~ 4 cells / well into 96-well plates were cultured in complete 1640 medium containing γTNF-α complete medium or γ-LT containing medium culture 3,5 Or 7 days.