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目的建立一种灵敏、简单测定生物样品中阿立哌唑与氯氮平的液相色谱-质谱联用法。方法样品处理采用液-液乙酸乙酯萃取方法。色谱柱为Zorbax SB-C18(2.1 mm×150 mm,5μm),流动相为乙腈与0.1%甲酸,梯度洗脱,流速为0.4 ml/min,柱温30℃。采用ESI离子源,MRM离子方式监测。结果阿立哌唑与氯氮平在50~2 000ng/m L浓度范围内线性良好(r>0.993);回收率均在87%~112%范围内,日内、日间RSD均小于12%。结论本方法可简单、高效地检测血液与尿液中阿立哌唑与氯氮平浓度。
Objective To establish a sensitive and simple method for the determination of aripiprazole and clozapine in biological samples by liquid chromatography-mass spectrometry. Methods Samples were processed using liquid-liquid ethyl acetate extraction. The column was Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm). The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient of 0.4 ml / min and a column temperature of 30 ° C. Using ESI ion source, MRM ion monitoring. Results Aripiprazole and clozapine showed a good linearity (r> 0.993) within the range of 50-2 000 ng / mL. The recoveries ranged from 87% to 112%. The RSDs were less than 12% in both days and days. Conclusion This method can be simple and efficient detection of blood and urine aripiprazole and clozapine concentrations.