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植物名称:甜茶(Rubus suavissimus)。材料类别:嫩茎和实生苗。培养条件:基本培养基为MS,培养实生苗的培养基不加任何激素;分化培养基附加 BA0.5—1.0mg/l(单位下同),GA_31.0;继代培养附加BA0.2—0.5,GA_3 1.0:诱导生根为1/2MS+IBA0.25—0.50。白糖3％,琼脂0.5％,pH为5.8。培养温度25±2℃;每天照光9—10小时,光照度约2000lx。生长和分化:实生苗培养:种子经消毒,破口
Plant name: sweet tea (Rubus suavissimus). Material categories: tender stems and seedlings. Culture conditions: the basic medium for the MS, the seedling culture medium without any hormone; differentiation medium added BA0.5-1.0mg / l (the same below), GA_31.0; subculture additional BA0.2- 0.5, GA_3 1.0: inducing rooting is 1 / 2MS + IBA0.25-0.50. 3% sugar, 0.5% agar, pH 5.8. Culture temperature 25 ± 2 ℃; daily light 9-10 hours, light intensity of about 2000lx. Growth and differentiation: Seedling culture: Seeds are sterilized and broken