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目的:观察活血通腑方优化方对腹腔粘连的治疗效果,确定活血通腑方优选方的最佳有效剂量。方法:取48只雄性SD大鼠随机分为假手术组、模型组、活血通腑方优化方2,6,18 g·kg-1组、阳性对照四磨汤(5.4 g·kg-1)组,除假手术组外,其余动物均采用锉刀法制备腹腔粘连大鼠模型,于术后第1天各组分别予以相应剂量的药物灌胃,假手术组给予等量的生理盐水,连续给药1周。术后7 d通过观察大鼠一般情况及腹腔粘连评分,酶联吸附法(ELISA)测定血清白介素-8(IL-8)、白介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)含量,同时实时荧光定量(RT-PCR)检测粘连组织中Ⅰ型胶原(Col-Ⅰ)mRNA的表达。结果:与假手术组相比,模型组肠壁各层血管高度扩张、水肿,浆膜层明显有淋巴细胞浸润、纤维母细胞增生;与模型组相比,各治疗组粘连处肠壁组织形态学上肌层结构完好,浆膜层轻度增厚,浆膜面纤维结缔组织增生不明显。与模型组相比,活血通腑方优化方中剂量组血清IL-8和TNF-α水平分别为(154.2±16.2),(58.2±6.4)ng·L-1,但是IL-10水平(58.2±6.4)ng·L-1升高,同时降低粘连组织中ColⅠmRNA的表达(0.41±0.11),有显著性统计学意义(P<0.05或P<0.01)。四磨汤组亦能降低腹腔粘连评分及血清IL-8和TNF-α水平,升高IL-10水平,但与活血通腑方优化方中剂量差异明显。结论:活血通腑方优化方中剂量组可通过调节IL-8,IL-10和TNF-α的水平抑制炎症反应,同时减少细胞外基质ColⅠmRNA的沉积,有效减轻腹腔粘连的程度。
Objective: To observe the therapeutic effect of Huoxue Tongfu Decoction on peritoneal adhesion and determine the optimal effective dose of Huoxue Tongfu prescription. Methods: Forty-eight male Sprague-Dawley rats were randomly divided into sham-operated group, model group, 2, 6 and 18 g · kg-1 groups of Huoxuetongfukang Formula and 5.4 g · kg- In addition to the sham-operation group, the other animals used the file method to prepare the model of peritoneal adhesions in rats. On the first day after operation, the rats in each group were given the corresponding dose of gavage, the sham operation group was given the same amount of saline, Medicine for 1 week. The levels of serum IL-8, IL-10 and TNF-α were detected by ELISA and ELISA at 7 days after operation. α), and the expression of collagen type Ⅰ (Col-Ⅰ) mRNA was detected by real-time fluorescence quantitative PCR (RT-PCR). Results: Compared with the sham operation group, the blood vessels in the intestinal wall of the model group were highly expanded, edematous and serosal layer obviously infiltrated with lymphocytes and fibroblast proliferation. Compared with the model group, the intestinal wall tissue morphology Learning muscular structure intact, mild serosal thickening, serosal surface fibrous connective tissue proliferation is not obvious. Compared with the model group, the serum levels of IL-8 and TNF-α in Huoxue Tongfu Decoction group were (154.2 ± 16.2) and (58.2 ± 6.4) ng · L -1, respectively, but the levels of IL-10 ± 6.4) ng · L-1, and decreased the expression of ColⅠmRNA in the adhesion tissue (0.41 ± 0.11) (P <0.05 or P <0.01). Si mill decoction group can also reduce the peritoneal adhesion score and serum levels of IL-8 and TNF-α, increased IL-10 levels, but with Huoxuetongfang prescription square dose significantly different. CONCLUSION: Huoxuetongfukang medium dose group can inhibit the inflammatory reaction by regulating the levels of IL-8, IL-10 and TNF-α, reduce the deposition of extracellular matrix ColⅠmRNA, and effectively reduce the degree of abdominal adhesions.