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作者报道在非霍乱流行区瑞典志愿者中进行口服霍乱B亚单位/全细胞(B-WC)菌苗的研究。该菌苗每剂含1.0mg纯化霍乱毒素B亚单位和1×10~(11)个死菌(含有稻叶、小川血清型及古典和E1Tor生物型霍乱弧菌株)。抗霍乱毒素的IgA和IgG抗体应答用ELISA法测定,抗菌抗体用微量杀弧菌法测定,抗毒素滴度提高2倍以上或杀弧菌抗体滴度提高4倍以上判为血清阳转。 第一项研究是比较口服B-WC菌苗2剂与3剂后的抗体应答。口服2剂及3剂后分别有97％和100％的志愿者IgA抗毒素滴度提高2倍以上,平均滴度分别增加20倍和
The authors report the study of oral cholera B subunit / whole cell (B-WC) vaccine in Swedish volunteers in non-cholera endemic areas. The vaccine contains 1.0mg of purified cholera toxin B subunit and 1 × 10-11 dead bacteria (containing rice leaf, Ogawa serotype and classical and E1Tor biotype Vibrio cholerae) per dose. The anti-cholera toxin IgA and IgG antibody responses were measured by ELISA. Antibacterial antibodies were assayed by the method of micro-Vibrio parahaemolyticus. The antitoxin titer was increased by 2-fold or the titer of the Vibrio cholerae antibody was increased by 4-fold. The first study compared antibody responses after oral administration of B-WC vaccine to two and three doses. After oral administration of 2 and 3 doses, IgA antitoxin titers were increased more than 2-fold in 97% and 100% of volunteers, respectively, and the average titer increased by 20-fold and