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采用无搅动原位聚合模式,在聚醚醚酮柱管中直接制备了聚合物整体固定相。通过扫描电镜观察到该整体固定相的孔径分布呈双峰模式,且孔结构均匀。用压汞法测定了该固定相的孔径分布、孔隙率及比表面积等参数,考察了致孔剂组成、聚合温度及交联剂含量等参数对固定相孔结构的影响,并对制备条件进行了优化。测定了流速与柱前压的关系,实验表明此整体固定相具有良好的通透性。通过对山羊血清和低聚核苷酸的分离分析,证明了所制备的整体固定相适合用于生物大分子的分离纯化。
Using agitation-free in-situ polymerization mode, the polymer monolith was directly prepared in polyetheretherketone column. The pore size distribution of the monolithic stationary phase showed a bimodal pattern by scanning electron microscopy and the pore structure was uniform. The pore size distribution, porosity, specific surface area and other parameters of the stationary phase were determined by mercury intrusion porosimetry. The effects of pore structure, polymerization temperature and crosslinking agent content on the pore structure of the stationary phase were investigated. The preparation conditions Optimized. The relationship between the flow rate and the pre-column pressure was measured. Experiments show that the monolithic stationary phase has good permeability. The separation and analysis of goat serum and oligonucleotide proved that the prepared monolithic stationary phase was suitable for the isolation and purification of biological macromolecules.