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为了探讨鱼藤酮对黑腹果蝇Drosophila melanogaster运动行为的影响与其头部多巴胺水平之间的关系,我们测定了鱼藤酮对黒腹果蝇成虫运动行为、头部多巴胺水平及酪氨酸羟化酶和多巴脱羧酶基因表达的影响。结果表明:与取食未加入药剂饲料的果蝇相比,雌成虫用0.2～0.8mmol/L、雄成虫用0.1～0.8mmol/L浓度药液配制的饲料连续饲养6d后运动能力显著下降,在0.8mmol/L浓度下雌、雄成虫的运动能力分别仅为对照的55.6%和49.1%。取食用0.8mmol/L浓度药液配制饲料6,12和21d的果蝇雌、雄成虫头部多巴胺水平均显著下降,雌成虫头部多巴胺水平分别为对照雌成虫的83.2%,72.3%和59.8%;雄成虫头部多巴胺水平分别为对照雄成虫的79.3%,66.8%和53.2%。用0.8mmol/L浓度鱼藤酮处理6,12和21d,雌成虫头部酪氨酸羟化酶基因(pale)的表达水平分别为对照的76.3%,51.4%和37.3%,多巴脱羧酶基因(Ddc)的表达水平分别为对照的87.1%,78.2%和63.5%,均显著下降。结果提示,鱼藤酮可干扰果蝇成虫头部酪氨酸羟化酶和多巴脱羧酶基因的表达,导致果蝇头部多巴胺水平下降,进而影响了果蝇的运动行为。
In order to investigate the relationship between rotenone on Drosophila melanogaster locomotor activity and its dopamine level in the head, we measured the effects of rotenone on adult behavior, head dopamine and tyrosine hydroxylase levels in Drosophila melanogaster Effect of Pata Decarboxylase Gene Expression. The results showed that compared with the fruit flies fed on the non-medicated feed, the ability of female adults to exercise continuously decreased significantly after being continuously fed for 6 days with 0.2-0.8 mmol / L and 0.1-0.8 mmol / L of male adult feed, At the concentration of 0.8mmol / L, the male and female adult motility were only 55.6% and 49.1% of the control respectively. The head dopamine levels of female and male adults were significantly decreased after feeding on the 6th, 12th and 21st day of feed with the concentration of 0.8mmol / L, and the levels of dopamine in female adults were 83.2%, 72.3% and 59.8 %; Male adult head dopamine levels were 79.3%, 66.8% and 53.2% of control male adults respectively. The expression level of tyrosine hydroxylase gene (pale) of female adult head was 76.3%, 51.4% and 37.3% respectively when treated with 0.2 mmol / L rotenone for 6, 12 and 21 d, respectively. The expression of dopa decarboxylase gene Ddc) expression levels were 87.1%, 78.2% and 63.5% of the control, respectively, were significantly decreased. The results suggest that rotenone can interfere with Drosophila adult head tyrosine hydroxylase and dopa decarboxylase gene expression, resulting in Drosophila head dopamine levels, thereby affecting the movement of Drosophila.