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以烟草原生质体为材料,采用彗星电泳检测用0.5W·m-2紫外线以不同时间(0、5、10、30、60和120s)诱导的烟草原生质体中DNA的损伤。结果表明,在0～10s的时间内代表DNA损伤程度的尾矩、Olive尾矩等参数与紫外线照射时间具有良好的时间依赖关系。本文建立的烟草原生质体体系采用彗星电泳技术,可以快速而灵敏地检测紫外线对植物细胞的损伤程度。
Tobacco protoplasts were used as materials to detect the damage of DNA in tobacco protoplasts induced by 0.5W · m-2 UV light at different times (0, 5, 10, 30, 60 and 120s) by comet assay. The results showed that the tail moment, Olive tail moment and other parameters representing the extent of DNA damage in 0 ~ 10s had a good time-dependent relationship with the time of UV irradiation. The tobacco protoplast system established in this paper adopts comet electrophoresis technology, which can quickly and sensitively detect the degree of UV damage on plant cells.