【摘 要】
:
为检测细胞培养用品对光透射率的影响,采用紫外分光光度计对培养瓶(板)、培养液、DHanks液等的透射规律和光敏剂的吸收规律进行观察。结果:对培养板(瓶),400nm~800nm光可透过80%以上,200nm~300nm光完全不能透过。RPMI1640细
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为检测细胞培养用品对光透射率的影响,采用紫外分光光度计对培养瓶(板)、培养液、DHanks液等的透射规律和光敏剂的吸收规律进行观察。结果:对培养板(瓶),400nm~800nm光可透过80%以上,200nm~300nm光完全不能透过。RPMI1640细胞培养液可让600nm~800nm光透过近100%,在575nm附近出现明显波谷,光透射率低,可能系酚红的影响;无酚红的DHanks液对210nm~800nm光透射率无明显影响。建立了一种培养细胞接受到的激光剂量的测定计算方法。
In order to detect the influence of cell culture articles on light transmittance, the transmission law of the culture flasks (plates), culture solution, D-Hanks solution and the absorption law of the photosensitizer were observed by UV spectrophotometer. Results: The plate (bottle), 400nm ~ 800nm more than 80% of the light can be transmitted, 200nm ~ 300nm light completely impermeable. RPMI1640 cell culture solution can let 600nm ~ 800nm light through nearly 100%, near 575nm obvious trough, light transmittance is low, may be the impact of phenol red; red phenol-free D Hanks solution of 210nm ~ 800nm light transmittance No significant effect. A method for calculating the laser dose received by cultured cells was established.
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