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应用PCR技术结合Southernblot分子杂交检测87例胃癌活检组织CerbB－2及K－sam扩增。结果显示：87例中有18例（20．69％）C－erbB－2扩增阳性，其中进展期胃癌、乳头状腺癌及分化良好型胃癌阳性率分别显著高于早期胃癌、其它组织类型胃癌及分化不良型胃癌（P均＜0.05）；伴淋巴结转移及肝转移胃癌阳性率显著高于不伴淋巴结及肝转移者（P＜0．01和P＜0．05）。5例胃癌K－sam扩增阳性，仅见于分化不良型胃癌（P＜0．05）。该结果可作为术前胃癌估计预后的一项指标。
The amplification of CerbB-2 and K-sam in 87 cases of gastric cancer biopsy were detected by PCR and Southern blot. The results showed that among 87 cases (18.69%), C-erbB-2 amplification was positive, and the positive rates of advanced gastric cancer, papillary adenocarcinoma, and differentiated gastric cancer were significantly higher than those of early gastric cancer and other tissue types, respectively. Gastric cancer and poorly differentiated gastric cancer (P <0.05); The positive rate of gastric cancer with lymph node metastasis and liver metastasis was significantly higher than those without lymph node and liver metastasis (P <0.01 and P <0.05). Five cases of gastric cancer were positive for K-sam amplification and were only found in poorly differentiated gastric cancer (P<0.05). This result can be used as an index to estimate the prognosis of preoperative gastric cancer.