作者采集682份已知或可疑丙型肝炎病毒(HCV)感染者的新鲜血清/血浆标本,用第2代ELISA和免疫印迹技术检测HCV抗体,用套式聚合酶链反应(PCR)检测HCVRNA,将血清学与PCR检测结果进行比较,以评价两者在HCV感染诊断中的一致性。对38例血浆HCV RNA阴性者,分离其外周血单个核细胞(PBMC),并检测HCV The authors collected fresh serum / plasma samples from 682 patients with known or suspected hepatitis C virus (HCV) infection. HCV antibodies were detected by second-generation ELISA and Western blotting. HCV RNA was detected by nested polymerase chain reaction (PCR) Serological and PCR test results were compared to evaluate the consistency of the two in the diagnosis of HCV infection. Thirty-eight patients with HCV RNA-negative plasma were isolated from peripheral blood mononuclear cells (PBMCs) and HCV