论文部分内容阅读
二倍体马铃薯基因组相对简单,借助二倍体进行育种可以加速马铃薯的育种进程,因此评价二倍体马铃薯种质的遗传多样性,挖掘和有效利用优良性状显得非常必要。为了筛选多态性的SSR标记,用55对SSR引物扩增39个遗传关系相对较远的二倍体马铃薯材料。选取分布在12条染色体上的12个具有高多态性的SSR标记评价192份二倍体马铃薯栽培品种的遗传多样性,共检测到98个等位位点,其中97个为多态性位点;每对SSR引物扩增出的等位位点为6~18个,平均8.2个。用非加权配对算术平均法(UPGMA)进行聚类,显示出所有供试材料的遗传关系:12对SSR引物可以将192份材料中的186份区分开;这192份材料被划分为11个组群,其中第一个组群包含了83.3%的材料。
The diploid potato genome is relatively simple. With the help of diploid breeding, the process of potato breeding can be accelerated. Therefore, it is necessary to evaluate the genetic diversity of diploid potato germplasm and to exploit and utilize the excellent traits. To screen polymorphic SSR markers, 55 pairs of SSR primers were used to amplify 39 diploid potato materials that were relatively distant from each other. Twelve microsatellite markers with high polymorphism distributed on 12 chromosomes were used to evaluate the genetic diversity of 192 diploid potato cultivars. A total of 98 alleles were detected, of which 97 were polymorphic Point; each pair of SSR primers amplified alleles 6 to 18, an average of 8.2. Clustering with Unweighted Pairwise Arithmetic Average (UPGMA) showed a genetic relationship for all tested materials: 12 pairs of SSR primers could distinguish 186 of 192 materials; these 192 materials were divided into 11 groups The first group consisted of 83.3% of the material.