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本文报道了用同基因脾细胞和抗原在体外不断再刺激天花粉蛋白免疫过的C57BL/6J小鼠的T淋巴细胞,能刺激自身反应性的T细胞在体外增殖并长期存活。实验结果表明它们的增殖是依赖于同基因脾细胞的再刺激,C57BL/6J(H一2~b),B 10 ScSn(H-2~b)和129(H-2~b)小鼠的脾细胞都能引起它们明显的增殖,但对C3H/He(H-2~K)和Balb/c(H-2~b)小鼠的脾细胞很弱,说明识别的可能是H-2~b抗原。应用未经免疫的C 57 BL/6 J小鼠的脾和淋巴结T淋巴细胞,采用同样的体外刺激方法,未能引起它们对同基因脾细胞的增殖。从而提示自身反应性T细胞是存在于正常机体内的一种能识别自身抗原的T淋巴细胞。在无外来抗原刺激时,它们可能是处于静止或不激活状态;在外来抗原诱发免疫过程中,它们也随了抗原特异的淋巴细胞一同被激活,并可能起调节作用。它们在免疫系统中的地位还有待进一步阐明。
In this paper, T lymphocytes of C57BL / 6J mice immunized with trichosanthin were continually re-stimulated with syngeneic splenocytes and antigens in vitro. T cells stimulating autoreactive T cells proliferated in vitro and survived in vitro. The experimental results show that their proliferation is dependent on the re-stimulation of syngeneic splenocytes, and the C57BL / 6J (H-2 ~ b), B10ScSn (H-2 ~ b) and 129 (H-2 ~ b) mice Spleen cells can cause their significant proliferation, but the spleen cells of C3H / He (H-2 ~ K) and Balb / c (H-2 ~ b) mice are weak, indicating that the identification may be H- b antigen. Splenocytes and lymph node T lymphocytes from non-immunized C57BL / 6J mice, using the same in vitro stimulation method, failed to induce their proliferation of syngeneic splenocytes. Suggesting that autoreactive T cells are T lymphocytes that recognize autoantigens present in the normal body. In the absence of foreign antigen stimulation, they may be in a quiescent or inactive state; they are also activated with antigen-specific lymphocytes and may play a regulatory role during foreign antigen-induced immunity. Their status in the immune system remains to be elucidated.