近年来随着病理细胞学进展,在涂片技术上不断有所改进,我们利用脑病变组织推片做术中快速诊断,即不影响病人的痛苦,又大大地缩短了确诊时间,现介绍如下:1.手术开颅后取一小块脑肿瘤或病变组织,或用穿刺针抽吸少许深部肿瘤组织,放于载玻片上。2.用另一张玻片轻压组织,然后呈45度角向前轻轻推移,用此方法连推三张。3.推片完毕后,立即置于95%酒精内固定约半分钟。4.用苏木素—伊红染色,封固后即可镜检。以上 In recent years, with the progress of pathology and cytology, smear technology continues to improve, we use the brain lesions to promote rapid intraoperative diagnosis, that does not affect the patient’s pain, but also greatly reduces the time of diagnosis, are introduced as follows : 1. After craniotomy surgery take a small piece of brain tumor or diseased tissue, or use a small needle aspiration of deep tumor tissue, placed on a glass slide. 2. With another slide gently press the organization, and then gently forward a 45-degree angle, with this method even push three. 3. Push the piece is completed, immediately placed in 95% alcohol for about half a minute. 4. With hematoxylin-eosin staining, sealing can be microscopic examination. the above