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目的探讨促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞的保护作用。方法体外传代培养大鼠视网膜Müller细胞,分组为N组(正常对照),G组(25 mmol/L葡萄糖),E1G组(1×104 IU/L rh EPO+25 mmol/L葡萄糖),E2G组(2×104 IU/L rh EPO+25 mmol/L葡萄糖),E4G组(4×104 IU/L rh EPO+25 mmol/L葡萄糖),MTT比色法比较五组视网膜Müller细胞活力的改变。酶联免疫吸附试验检测各组细胞caspase-3蛋白表达的情况。结果 G组、E1G组、E2G组、E4G组细胞活力均低于N组,E1G组、E2G组、E4G组细胞活力高于G组,E4G组高于E2G组,E2G组高于E1G组。酶联免疫吸附试验检测结果显示G组caspase-3表达高于N组,rh EPO干预各组caspase-3表达均低于G组,并随EPO干预浓度增高表达降低,但均高于N组。结论促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞有保护作用。
Objective To investigate the protective effect of erythropoietin on retinal Müller cells cultured in high glucose. Methods Rat retinal Müller cells were subcultured and divided into N groups (normal control), G group (25 mmol / L glucose), E1G group (1 × 104 IU / L rh EPO + 25 mmol / L glucose) (2 × 104 IU / L rh EPO + 25 mmol / L glucose) and E4G group (4 × 104 IU / L rh EPO + 25 mmol / L glucose). MTT assay was used to compare the changes of vitality of Müller cells in five groups. Enzyme-linked immunosorbent assay was used to detect caspase-3 protein expression in each group. Results The cell viability in G group, E1G group, E2G group and E4G group was lower than that in N group. The cell viability in E1G group, E2G group and E4G group was higher than that in G group, higher in E4G group than in E2G group, higher in E2G group than in E1G group. The results of enzyme-linked immunosorbent assay showed that the expression of caspase-3 in G group was higher than that in N group. The expression of caspase-3 in rhEPO-treated group was lower than that in G group, and decreased with the increase of EPO concentration. Conclusion Erythropoietin has a protective effect on cultured rat retinal Müller cells cultured with high glucose.